The cyclin T1 subunit of the HIV Tat cofactor TAK/P-TEFb is transiently induced during macrophage differentiation.
About the Lab
The Rice laboratory studies how gene expression is regulated in HIV - the virus that causes AIDS. The major focus of our research is on the HIV Tat protein, which is essential for HIV transcription, and on two cellular factors, cyclin T1 and Cdk9, which are necessary for Tat function. We are investigating how cyclin T1 and Cdk9 are regulated in the two cell types that HIV infects - CD4+ T cells and macrophages.
On this web site, you will find short summaries of several research projects that are ongoing in the laboratory, information about members of the lab, and some of our laboratory protocols.
Protocols
Data
- From TL Sung, L Golebiewski, E Siwak, and AP Rice. (Manuscript submitted).
Sung TL, Rice AP. PLoS Pathog. 2009 Jan;5(1):e1000263. Epub 2009 Jan 16. .
Microarray data - from TL Sung and AP Rice. (2006) Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+ T cells. - Retrovirology. 3(1):66.
- Yu, W., Wang, Y., Shaw, C., Qin, X.-F., and Rice, A.P. (2006) Induction of HIV-1 Tat co-factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs. Retrovirology 3:32.
- from R.E. Haaland, W. Yu, and A.P. Rice. (2005) Identification of LKLF-regulated genes in quiescent CD4+ T lymphocytes - Molecular Immunology, 42:627-641.
- from Haaland, R.E., Herrmann, C.H., and Rice, A.P. (2005). siRNA depletion of 7SK snRNA induces apoptosis but does not affect expression of the HIV-1 LTR or P-TEFb-dependent cellular genes. J. Cell. Physiol. 205:463-470.
Resources
*The GST-Tat constructs made in our lab are available through this program.
